Vitamin B2
Description: 
Whole-blood vitamin B2 (flavin adenine dinucleotide (FAD)) is measured by HPLC with fluorescence detection
Due to the relocation of the Nutristasis service to the Blackfriars Hub site, this test is not currently accredited by UKAS to ISO15189
Due to the relocation of the Nutristasis service to the Blackfriars Hub site, this test is not currently accredited by UKAS to ISO15189
Clinical details: 
The term ‘vitamin B2’ indicates three compounds with vitamin B2 activity found in nature; flavin adenine dinucleotide (FAD), flavin mononucleotide (FMN) and riboflavin. Vitamin B2 can be found in most unprocessed foods and at high concentrations in yeast extract liver and kidney. Meat, milk eggs and wheat bran are important dietary sources of vitamin B2 in the general population. 
Because of its wide distribution in foods, symptoms of vitamin B2 deficiency are hard to distinguish in malnourished individuals due to concomitant deficiencies in other micronutrients. Experimentally, signs of deficiency are lesions of the lips (cheilosis), angles of the mouth (angular stomatitis), a fissured and magenta coloured tongue (glossitis) and follicular keratosis of the nose and forehead. The eyes can also be effected, symptoms include corneal vascularisation and photophobia.
Because of its wide distribution in foods, symptoms of vitamin B2 deficiency are hard to distinguish in malnourished individuals due to concomitant deficiencies in other micronutrients. Experimentally, signs of deficiency are lesions of the lips (cheilosis), angles of the mouth (angular stomatitis), a fissured and magenta coloured tongue (glossitis) and follicular keratosis of the nose and forehead. The eyes can also be effected, symptoms include corneal vascularisation and photophobia.
Reference range: 
174-471
Synonyms or keywords: 
Riboflavin; flavin adenine dinucleotide (FAD), flavin mononucleotide (FMN) 
Units: 
nmol/L
Department: 
Location: 
Sample type and Volume required: 
Minimum sample volume = 500 μL of EDTA whole blood 
Call in advance: 
No
Turnaround time: 
10 days
Special sample instructions: 
Protect specimens from light and store frozen at -20 degrees.
Storage and transport: 
Transport at room temperature
Contacts:
								Nutristasis Unit at Blackfriars Hub
0204 591 0055
Nutristasis@synnovis.co.uk
Reference Chemistry, 1st Floor Blackfriars Hub
Friars Bridge Court
Blackfriars Road
London
SE1 8NZ
Laboratory opening times
Monday - Friday 09.00 - 17.00
Friars Bridge Court
Blackfriars Road
London
SE1 8NZ
Laboratory opening times
Monday - Friday 09.00 - 17.00
Laboratory: 
Last updated: 22/10/2025

