Activated protein C resistence screening

Determination of activated protein C resistence using Pefakit APC-R Factor V Leiden kit. Pefakit® APC-R Factor V Leiden is a plasma-based functional clotting assay. The assay is highly specific for FV mutations that confer APC resistance as there is no interference by direct-Xa inhibitors, lupus anticoagulants, the effects of VKA anticoagulation, and abnormal conditions of fibrinogen, FVIII, FX, or D-Dimer. Interference from UFH and LMWH in the plasma sample is largely precluded by a heparin neutraliser integral to reagents 1 and 2. Direct thrombin inhibitors are a potential interference.
Clinical details: 
Activated protein C resistance (APCR) is defined as a poor anticoagulant response of plasma to APC and has both hereditary and acquired causes. More than 95% of hereditary cases are due to the FV Leiden point mutation (changing arginine at 506 in FV gene to glutamine). This results in an impaired inactivation rate of FVa due to alteration of a protein C cleavage site. Native FVa is an important procoagulant co-factor to FXa in the prothrombinase complex and the result of the mutation is that thrombin formation will not be stopped as efficiently as in the case of inactivation of native FVa. Compared to normal FV genotype, the heterozygous defect is associated with 5 to 10 fold and homozygous defect with a 50 to 100 fold increased thrombosis risk. Other factor V mutations include FV Cambridge and FV Hong Kong. No mutations in the FVIII gene conferring APCR have been described. Acquired APCR has been described in pregnancy, patients with high FVIII levels, patients with anti-phospholipid antibodies, and is associated with use of the oral contraceptive pill.
Reference range: 

APCR ratio 3.0 – 7.0

Synonyms or keywords: 
Sample type and Volume required: 
External requests: Citrated platelet poor plasma 500µL x 1 aliquot
Internal requests: please refer to EPR label
Turnaround time: 
7 - 12 days
Special sample instructions: 

The sample should be analysed or processed & stored within 4 hours of venepuncture. Please ensure sample tubes are filled exactly to the fill-line as underfilling or overfilling creates a dilution error and leads to inaccurate results.

Storage and transport: 
It is advised that citrated plasma is frozen prior to transport and sent to the laboratory on dry ice to maintain sample quality and integrity.
Diagnostic Haemostasis and Thrombosis Department
St Thomas': 020 7188 2797; Guy's: 020 7188 7188 ext. 53860
St Thomas' Hospital
North Wing - 4th and 5th Floors
Westminster Bridge Road
London SE1 7EH

Laboratory opening times

Guy's Hospital
Southwark Wing - 4th Floor
Great Maze Pond
London SE1 9RT

Outside core hours, contact Duty Haemostasis Biomedical Scientist
For clinical advice or interpretation of results, please contact the laboratory in the first instance.

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Last updated: 08/06/2023