Anti-Etanercept Antibodies (Free)

The measurement of anti-Etanercept antibodies enables a greater understanding as to the underlying aetiology of treatment failure. Patients with detectable anti-Etanercept antibodies may be at risk of developing hypersensitivity reactions, particularly those being re-introduced after drug interruption. The detection of anti-Etanercept antibodies also guides management decisions on concomitant immunomodulation, dose escalation, drug switching and withdrawal.

Measured as part of a profile including Etanercept levels.

This test is not currently included in the laboratory's UKAS scope of accreditation to ISO15189:2012.
Clinical details: 
Due to the structure of the anti-TNFα drugs, the immune system may stimulate formation of antibodies against the drug (ADA, anti-drug antibodies). Antibody formation is associated with allergic reactions and loss of response due to neutralisation of biological effect and/or increased clearance of drug. Etanercept, being a fully humanised fusion protein, is thought to be less immunogenic than Infliximab.

Reference range: 

Test interpretation requires full clinical information: drug dosage/frequency, previous exposure to other biologics, time of sampling, duration of therapy and clinical assessment. <5 ng/mL

Sample type and Volume required: 
Serum (SST tube or plain) sample is preferred. Plasma (Lithium heparin, citrate or EDTA) is also acceptable. Minimum 300μL serum or plasma required for both drug and anti-drug antibody analysis.
Turnaround time: 
Please contact the laboratory to discuss.
Special sample instructions: 

Collect blood into a serum separation (SST™) or plain tube, preferably shortly before drug administration (trough levels). Centrifuge sample at 3000 rpm for 10 minutes, aliquot serum or plasma and keep in fridge until transport. If transport is going to be delayed by > 5 days, freeze at -20°C and send by first class post at a later date.

Storage and transport: 
Post the sample to Viapath Central Specimen Reception by first class post (see request form for full address).
Reference Chemistry Laboratory at St Thomas'
020 7188 1264
4th floor, North Wing
St Thomas' Hospital
Westminster Bridge Road
London SE1 7EH
For clinical advice or interpretation of results, please contact the laboratory in the first instance.

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Last updated: 12/10/2022