Anti Neutrophil Cytoplasmic Antibody (ANCA) (MPO & PR3)

Anti-MPO (myeloperoxidase) and PR(proteinase)3 assays are performed on serum which are ANCA positive by indirect immunofluorescence. There may also be clinical grounds for performing these investigations on ANCA negative sera as ANCA negative but anti-MPO/PR3 positive vasculitis can occur.

Anti-MPO is primarily associated with pauci-immune crescentic glomerulonephritis, eosinophilic granulomatosis with polyangitis (Churg-Strauss syndrome) and microscopic polyangiitis. Anti-PR3 is associated with granulomatosis with polyangitis (Wegener’s granulomatosis). However, the fore mentioned associations are not exclusive.
Reference range: 

anti MPO antibodies <3.5
anti PR3 antibodies <1.9

Sample type and Volume required: 
Clotted (Gold top vacutainer) 4 ml
Turnaround time: 
2 days
Special sample instructions: 

All tests are compromised by prolonged transit times. For this reason, date of bleed and date of postage must be provided with each request.

Storage and transport: 
Serum stored at 4°C should be sent by first class post
Time limit for extra tests: 
10 days
Diagnostic Immunology and Allergy Department at King's College Hospital
General Enquiry : 020 3299 8752 / 020 3299 1171
King's College Hospital
Bessemer Wing - 1st Floor
Denmark Hill
London SE5 9RS
For clinical advice or interpretation of results, please contact the laboratory in the first instance.

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Last updated: 12/06/2018