Anti Neutrophil Cytoplasmic Antibodies (ANCA),

Detection of anti-neutrophil cytoplasmic antibodies (ANCA) is important for the diagnosis and monitoring of ANCA-associated vasculitis (AAV) which includes granulomatosis with polyangitis (Wegener’s granulomatosis); microscopic polyangitis eosinophilic granulomatosis with polyangitis (Churg-Strauss syndrome).
This is an indirect immunofluorescence (IIF) assay using ethanol fixed neutrophils as substrate. A number of IIF patterns are recognised including perinuclear (P-ANCA), cytoplasmic (C-ANCA) and atypical ANCA.
C-ANCA is usually associated with anti-PR3 specificity. P-ANCA may be associated with anti-MPO specificity. Other antigens recognised by ANCA include lactoferrin, lysozyme, cathepsin G and bacterial/permeability increasing (BPI) enzyme and usually associated with atypical ANCA patterns.
Because the immunofluorescence pattern is not antigen specific all positive ANCA patterns are further investigated with antigen specific ELISA for both anti-myeloperoxidase (MPO) and anti-proteinase 3 (PR3) which are the usual clinically significant specificities.
Reference range: 


Negative / To confirm
Sample type and Volume required: 
Clotted (Gold top vacutainer)
Call in advance: 
If urgent
Turnaround time: 
3 days
Special sample instructions: 

All tests are compromised by prolonged transit times. For this reason, date of bleed and date of postage must be provided with each request.

Storage and transport: 
Serum stored at 4°C should be sent by first class post
Time limit for extra tests: 
10 days
Diagnostic Immunology and Allergy Department at King's College Hospital
General Enquiry : 020 3299 8752 / 020 3299 1171
King's College Hospital
Bessemer Wing - 1st Floor
Denmark Hill
London SE5 9RS
For clinical advice or interpretation of results, please contact the laboratory in the first instance.

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Last updated: 12/06/2018