17-Hydroxyprogesterone (17-OHP)

Measurement of 17a-hydroxyprogesterone is used in the diagnosis and management of patients with classical congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency. It is also useful in identifying and excluding late-onset forms of CAH in patients presenting with hirsutism and infertility. Method of analysis is solid phase extraction (SPE) following by liquid chromatography tandem mass spectrometry (LC-MS/MS). Please note, for a period of 6 months from 22nd May 2023 we will be reporting a 21-deoxycortisol result with every raised 17OHP, adding specificity to the 17OHP result. There will be no charge from Synnovis for this additional result. After this time, 21-deoxycortisol will revert to being a user requested, billable service. Please refer to 21-deoxycortisol [https://www.synnovis.co.uk/our-tests/21-deoxycortisol] for further information.
Clinical details: 
Deficiency of 21-hydroxylase activity leads to an increased concentration of the immediate precursor, 17a-hydroxyprogesterone, in the peripheral circulation. Inability of the adrenal glands to synthesise glucocorticoids and mineralocorticoids stimulates over-production of ACTH from the pituitary which in turn drives the production of adrenal androgens.

Note: Deficiency of 11b-hydroxylase activity also causes an increased concentration of 17a-hydroxyprogesterone in the peripheral circulation but measurement of 11-deoxycortisol should be used for this diagnosis.
Reference range: 

Male and female 0-5 days 0 - 3nmol/L.
Male and female 5 days to 16 years 0 - 4nmol/L.
Male 16 and over 0 - 5nmol/L.
Female 16 and over 0 - 5nmol/L - results may be higher in luteal phase
Post 250ug Synacthen stimulation
Normal at 30 mins < 10nmol/L

Sample type and Volume required: 
Clotted (yellow SST) preferred, but EDTA plasma also accepted. Volume required: 250uL; Absolute minimum required: 100uL.
Turnaround time: 
7 working days
Special sample instructions: 

In neonates the sample should be taken at least 48 hours post birth.

Storage and transport: 
Sample Stability: stable at 4°C for 25 days and up to 6 months at -20°C
Steroid Laboratory at King's College Hospital
020 3299 4131
King's College Hospital
Denmark Hill
London SE5 9RS
For clinical advice or interpretation of results, please contact the laboratory in the first instance.

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Last updated: 05/06/2023