Immunophenotyping for haematological malignancies

To diagnose and classify suspected haematological abnormalities involving Leucocytes. Immunophenotyping is offered to supplement and complement the services provided by cytogenetics and minimal residual disease as part of a comprehensive diagnostic service.

Immunophenotyping is carried out to assist in the diagnosis and monitoring of the broad spectrum of haematological malignancies, including but not limited to chronic lymphoproliferative diseases (LPD), acute myeloid leukemia (AML), acute lymphocytic leukemia (ALL) and plasma cell disorders.

A panel of antibodies will be selected based on the clinical information provided and if indicated by our initial morphology and flow cytometry findings further testing may be performed at the discretion of senior member of the Immunophenotyping team and /or haematology consultant. This system allows us to ensure appropriate and thorough investigations have been performed and reduce the need for further samples to be taken.
Synonyms or keywords: 
acute chronic leukaemia lymphoma immunophenotyping flow cytometry leukemia flowcytometry panel blasts AML ALL MRD CLL LPD lymphoproliferative disorder haemato-oncology myeloid lymphoid precursors haematological malignancy
Sample type and Volume required: 
Blood in EDTA (1ml minimum). Bone Marrow in EDTA or culture media.
Turnaround time: 
5 days (24 hours if notification of urgent request received)
Special sample instructions: 

Specimens should be delivered directly to the reception immediately after aspiration is performed on site. Where specimens are taken offsite the following storage conditions apply whilst awaiting transport to the laboratory:
- CSF specimens in Transfix should be stored between 2-8oC awaiting transport to the laboratory.
- Peripheral blood and bone marrow EDTA specimens and CSF and other fluid specimens in universal containers should be stored at room temperature if awaiting same day transport.  If transport is the arranged the following day (or over a weekend), PB and BM specimens should be stored between 2-8oC and fluids in universal containers should be stored at room temperature.

Storage and transport: 
Ideally, send by motorcycle courier (to avoid parking problems at King’s). If not urgent, send by overnight first class post. Samples will keep at refrigerated temperatures over a weekend if necessary.
Please contact Business Development for pricing enquiries
SE-HMDS Department at King's College Hospital
020 3299 9000 ext 32414
c/o Central Specimen Reception
Blood Sciences Laboratory
Ground Floor Bessemer Wing
King’s College Hospital
Denmark Hill
London SE5 9RS
Mon-Fri, 9.00am-5.30pm
For clinical advice or interpretation of results, please contact the laboratory in the first instance.

Print as a PDF

Last updated: 14/01/2021